Journal: bioRxiv
Article Title: Transcriptomic Analysis of the Newborn Mammary Gland Identifies Prenatal Ethanol-Induced Remodeling of Insulin Signaling and Enhanced Proliferative Programming
doi: 10.64898/2026.09.22.753596
Figure Lengend Snippet: (A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), IRS1, EGFR, ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, ERα, Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.
Article Snippet: Proteins examined included IRS1(#3407), phospho-IRS1 (Ser612) (#3203), phospho-IRS1 (Tyr895) (#3070), phospho-Akt1 (Ser473) (#4060), ErbB3 (#12708), phospho-ERK1/2 (Thr202/Tyr204) (#9101) were purchased from Cell signaling Technology, EGFR (sc-03), IGF1R (sc-712), Akt1 (sc-5298), Erk2 (sc-1647), ERα (sc-8002), Sox2 (sc-398254), IGFBP5 (sc-515116) and β-Actin (sc-47778) were purchased from Santa Cruz Biotechnology.
Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot, Analysis